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droplet generator  (Bio-Rad)


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    Structured Review

    Bio-Rad droplet generator
    Droplet Generator, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3877 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qx200+droplet+generator/QX200+Droplet+Generator/pmc13175773-263-6-8
    Average 96 stars, based on 3877 article reviews
    droplet generator - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Generated:

    Article Title: Combination treatment with synthetic gRNA/Cas12a and gRNA/Cas9 ribonucleoproteins disrupts HIV replication and expression.
    Article Snippet: Each 20 μL ddPCR reaction mix contained cDNA, 500 nM of primers, 250 nM of probe, and 2x ddPCR Supermix for Probes (Bio-Rad). .. The 20 μL reaction mix was loaded into DG8 cartridge with 70 μL of droplet generation oil (Bio-Rad), and droplets were generated using the QX200 Droplet Generator (Bio-Rad). .. 40 μL of droplets were transferred to a 96-well plate, and PCR was performed in a C1000 Touch Thermal Cycler (Bio-Rad) with the following cycling conditions: initial denaturation at 95 ◦C for 10 min; 40 cycles of 94 ◦C for 30 s and 60 ◦C for 60 s; and a final enzyme deactivation at 98 ◦C for 10 min, with a ramp rate of 2◦C/s.

    Article Title: Combination treatment with synthetic gRNA/Cas12a and gRNA/Cas9 ribonucleoproteins disrupts HIV replication and expression
    Article Snippet: Each 20 μL ddPCR reaction mix contained cDNA, 500 nM of primers, 250 nM of probe, and 2x ddPCR Supermix for Probes (Bio-Rad). .. The 20 μL reaction mix was loaded into DG8 cartridge with 70 μL of droplet generation oil (Bio-Rad), and droplets were generated using the QX200 Droplet Generator (Bio-Rad). .. 40 μL of droplets were transferred to a 96-well plate, and PCR was performed in a C1000 Touch Thermal Cycler (Bio-Rad) with the following cycling conditions: initial denaturation at 95 °C for 10 min; 40 cycles of 94 °C for 30 s and 60 °C for 60 s; and a final enzyme deactivation at 98 °C for 10 min, with a ramp rate of 2°C/s.

    Article Title: Safety and efficacy of allogeneic CD19-directed CAR-T therapy CTX110 in relapsed/refractory B-cell non-Hodgkin lymphoma.
    Article Snippet: Droplet digital 151 polymerase chain reaction (ddPCR) was prepared with 2X ddPCR Supermix (Bio-Rad 152 Laboratories, Hercules, CA, USA), FAM dye/MGB probe targeting the CAR insert (Thermo 153 Fisher Scientific [TFS], Waltham, MA, USA), VIC dye/QSY probe targeting reference gene 154 ABHD4 (TFS), deoxynucleoside triphosphates, and MgCl2. .. Droplets were generated 155 automatically with QX200 Droplet Generator (Bio-Rad Laboratories). ..

    Article Title: A Modular and Programmable Cas13d Platform for RNA Single Nucleotide Variant Detection.
    Article Snippet: Each reaction contained 10 μL ddPCR Supermix for Probes without dUTP from Bio-Rad, 1.8 μL of each primer at a concentration of 10 μM, 0.5 μL of each probe at a concentration of 10 μM, 4 μL of template, and 1.4 μL of nuclease-free water. .. Droplets were generated using DG8 cartridges and QX200 droplet generation oil from Bio-Rad on a QX200 Droplet Generator. ..

    Article Title: Unmasking the common enemy: drug resistance mechanisms across three different EGFR inhibitor generations are associated with co-targetable alterations in extracellular matrix signaling.
    Article Snippet: .. Droplets were generated in a total volume of 90 μL (25 ng cDNA/20 μL of reaction system and 70 μL of generation oil droplets) using the QX200 Droplet Generator (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s protocol. .. The generated droplets were carefully pipetted into 96-well PCR plates (40 μL/well) and sealed using the PX1 PCR Plate Sealer (Bio-Rad Laboratories, Hercules, CA, USA) at 180 ̊C.

    other:

    Article Title: Light-inducible CRISPR/Cas9 system for genome editing
    Article Snippet: Primers and probes were designed using guidelines by Bio-Rad (planning-droplet-digital-pcr-experiments).



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    Image Search Results


    Journal: bioRxiv

    Article Title: Molecular Star Gazing: Development and Validation of an Environmental DNA Assay for the Imperiled Sunflower Sea Star ( Pycnopodia helianthoides )

    doi: 10.64898/2026.05.07.723600

    Figure Lengend Snippet:

    Article Snippet: Nucleic-acid extracts were run as template (5 μl) in three replicate ddPCR reactions using the finalized ddPCR conditions (see Results) and an AutoDG Automated Droplet Generator (Bio-Rad, Hercules, CA), a C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA) and a QX200 Droplet Digital PCR System (Bio-Rad, Hercules, CA) ( ).

    Techniques:

    Proportion of samples or dive transects across the four regions with a positive detection of Pycnopodia by either underwater visual census (UVC) or by ddPCR assay of water samples (eDNA). Samples below LoD were set to zero.

    Journal: bioRxiv

    Article Title: Molecular Star Gazing: Development and Validation of an Environmental DNA Assay for the Imperiled Sunflower Sea Star ( Pycnopodia helianthoides )

    doi: 10.64898/2026.05.07.723600

    Figure Lengend Snippet: Proportion of samples or dive transects across the four regions with a positive detection of Pycnopodia by either underwater visual census (UVC) or by ddPCR assay of water samples (eDNA). Samples below LoD were set to zero.

    Article Snippet: Nucleic-acid extracts were run as template (5 μl) in three replicate ddPCR reactions using the finalized ddPCR conditions (see Results) and an AutoDG Automated Droplet Generator (Bio-Rad, Hercules, CA), a C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA) and a QX200 Droplet Digital PCR System (Bio-Rad, Hercules, CA) ( ).

    Techniques:

    eDNA concentrations measured by ddPCR assay after log10 transformation. Concentrations below the limit of detection were set to zero. Pycnopodia helianthoides biomass density at the transect level was calculated as kg per 10 m2 using a length–biomass conversion from . We find better alignment in eDNA and diver abundance estimates when averaging at larger scales as compared to point transect and bottle comparisons. Fjords are depicted in purple and Outer Islands are in orange.

    Journal: bioRxiv

    Article Title: Molecular Star Gazing: Development and Validation of an Environmental DNA Assay for the Imperiled Sunflower Sea Star ( Pycnopodia helianthoides )

    doi: 10.64898/2026.05.07.723600

    Figure Lengend Snippet: eDNA concentrations measured by ddPCR assay after log10 transformation. Concentrations below the limit of detection were set to zero. Pycnopodia helianthoides biomass density at the transect level was calculated as kg per 10 m2 using a length–biomass conversion from . We find better alignment in eDNA and diver abundance estimates when averaging at larger scales as compared to point transect and bottle comparisons. Fjords are depicted in purple and Outer Islands are in orange.

    Article Snippet: Nucleic-acid extracts were run as template (5 μl) in three replicate ddPCR reactions using the finalized ddPCR conditions (see Results) and an AutoDG Automated Droplet Generator (Bio-Rad, Hercules, CA), a C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA) and a QX200 Droplet Digital PCR System (Bio-Rad, Hercules, CA) ( ).

    Techniques: Transformation Assay